This file explains all of the variables in each of the datasets that accompany: Johnson et al. 2017 ms. Evidence that fertility trades off with early offspring fitness as males age
Cohort-based longitudinal analyses:
dataset: MaleSurvival.csv
Age = age of males when sampling (months)
Tank = tank fish housed in, ML6B1, ML6B2 or ML6B3
Survived = number of males alive at sampling
x.Survived = number of males dad at sampling (from total of 15)
P_Survived = proportion of males surviving
dataset: MatingSuccess.csvÊ
Age = age of males when sampling (months)
Tank = tank fish housed in, ML6B1, ML6B2 or ML6B3
Mated = number of surviving males that mated (i.e., fertilised eggs produced) during mating interval
x.Mated = number of surviving males that did not mate during mating interval
P_Mated = proportion of males that mated
dataset: SpermProduction.csv
Age = age of males when sampling (months)
Tank = tank fish housed in, ML6B1, ML6B2 or ML6B3
VOL = male ejaculate volume, categorised into 4 levels (0=no semen, 1=0-1 uL, 2=1-3 uL, 3=3+ uL)
VOL_binary = male ejaculate collected at sampling (0 = no, 1 = yes)
dataset: SpermConcentration.csv
Age = age of males when sampling (months)
Tank = tank fish housed in, ML6B1, ML6B2 or ML6B3
FishID_unique = male ID, a combination of the age at sampling, the line (ABZ) and the Fish ID
Sperm_mL = concentration of sperm per mL
Size = total male length (cm)
dataset: CASA.csv
Age = age of males when sampling (months)
Tank = tank fish housed in, ML6B1, ML6B2 or ML6B3
FishID_unique = male ID, a combination of the age at sampling, the line (ABZ) and the Fish ID
VAP = sperm swimming speed (actual path velocity)
VSL = sperm swimming speed (straight line velocity)
VCL = sperm swimming speed (curvilinear velocity)
MOTILE_PCT = percentage of motile sperm in sample
Size = total male length (cm)
dataset: IVF.csvÊ
Age = age of males when sampling (months)
Tank = tank fish housed in, ML6B1, ML6B2 or ML6B3
FishID_unique = male ID, a combination of the age at sampling, the line (ABZ) and the Fish ID
Developed = number of developed embryos at 24 hours post fertilisation
x.Developed = number of undeveloped embryos at 24 hours post fertilisation
P_Developed = sperm swimming speed (curvilinear velocity)
Hatch = number of developed embryos (@ 24 hpf) that hatched by 5 days
x.Hatch = number of developed embryos that did not hatch by 5 days
P_Hatch = proportion of developed embryos (@ 24 hpf) that hatched by 5 days
Size = total male length (cm)
dataset: ProgenySurvival.csvÊ
Age = age of males when sampling (months)
Tank = tank fish housed in, ML6B1, ML6B2 or ML6B3
FishID_unique = male ID, a combination of the age at sampling, the line (ABZ) and the Fish ID
Survived = number of developed embryos (@ 24hpf) that survived to 15 days
x.Survived = number of developed embryos (@ 24 hpf) that did not survive to 15 days
P_Survived = sperm swimming speed (curvilinear velocity)
Cross-sectional (young vs. old males) analyses:
dataset: CASA_CS.csv
Age = age of males when sampling (months)
FishID_unique = male ID
VAP = sperm swimming speed (actual path velocity)
VSL = sperm swimming speed (straight line velocity)
VCL = sperm swimming speed (curvilinear velocity)
MOTILE_PCT = percentage of motile sperm in sample
dataset: IVF_CS.csvÊ
Age = age of males when sampling (months)
FishID_unique = male ID
Developed = number of developed embryos at 24 hours post fertilisation
x.Developed = number of undeveloped embryos at 24 hours post fertilisation
P_Developed = sperm swimming speed (curvilinear velocity)
Hatch = number of developed embryos (@ 24 hpf) that hatched by 5 days
x.Hatch = number of developed embryos that did not hatch by 5 days
P_Hatch = proportion of developed embryos (@ 24 hpf) that hatched by 5 days
dataset: ProgenySurvival_CS.csvÊ
Age = age of males when sampling (months)
FishID_unique = male ID
Survived = number of developed embryos (@ 24hpf) that survived to 15 days
x.Survived = number of developed embryos (@ 24 hpf) that did not survive to 15 days
P_Survived = sperm swimming speed (curvilinear velocity)